Aim To investigate the genetic association of glutathione peroxidase-1（GPX-1） gene polymorphism in patients with kawasaki disease （KD）,and coronary artery lesions （CAL）. Methods There were 92 patients with KD and 108 healthy subjects in this study. The genotype of locus －46C/T and locus 599C/T of GPX-1 were detected by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）. Results For －46C/T polymorphism and 599C/T polymorphism in GPX-1 gene,there were no significant differences between KD patients and the controls in genotype frequencies of CC,CT and TT,and allele frequencies of C and T （χ²0.174 and 0.166,both P>0.05 χ²0.429 and 0.368,both P>0.05） For －46C/T polymorphism and 599C/T polymorphism in GPX-1 gene,there were no significant differences between KD patients with CAL and without CAL in genotype and allele frequencies of C and T （χ²0.507 and 0.487,both P>0.05 χ²0.635 and 0.535,both P>0.05）. Conclusion No association was found in －46C/T polymorphism and 599C/T polymorphism between GPX-1 gene and the risk of KD or its complication of CAL in this study.