Aggravating Atherosclerosis of the Fusion-Protein of CTB and Heat Shock 65 Epitopes by Subcutaneous Injection
Author:
  • Article
  • | |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • |
  • Cited by
  • | |
  • Comments
    Abstract:

    Aim To study the immunization effects of B epitopes of heat shock protein 65 on the atherosclerosis plaque formation of high fat diets animals. Methods By using two functional epitopes P1(179-190),P2(31-46)of HSP65 related to inflammatory autoimmune disease,high-level expression vector of pET28a-CTB-p117 was built with CTB as a carrier protein.The fusion protein of CTB-p117 was acquired by expression and purification process and used to immunize the animals by subcutaneous and intracutaneous injections. Results Anti-HSP65 antibodies were induced and both blood total cholesterol levels and aortic As plaques significantly increased compared with control group (P<0.05).Conclusions The HSP65 epitopes (CTB as the carrier protein) were able to aggravate As plaque formation after subcutaneous injections which could be the reasons that the induced anti-HSP65 antibodies promoted inflammatory reaction,lipid metabolic changes,and then accelerate the As plaque formation.The above HSP65 epitopes can be used to construct the anti-As vaccine to inhibit the As plaque formation by different immunization way.

    Reference
    Related
    Cited by
Get Citation

SUN Yun-Xiao, ZHANG Li-Biao, WANG Hua-Qian, ZHANG Yu, XIONG Qi-Yan, LI Tai-Ming, and CAO Rong-Yue. Aggravating Atherosclerosis of the Fusion-Protein of CTB and Heat Shock 65 Epitopes by Subcutaneous Injection[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2014,22(10):988-992.

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:December 05,2013
Article QR Code