Aim To investigate the relationship among scavenger receptor class B type Ⅰ （SR-BⅠ） single gene polymorphisms （exon 1 and intron 5）, the risk of coronary heart disease （CHD） and blood lipid concentration in Tianjin Han Chinese population. Methods SR-BⅠ exon 1 and intron 5 polymorphisms were genotyped in 370 CHD patients and 143 healthy adults undergoing coronary angiography by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）. The concentrations of blood lipid were examined in all subjects. Results Intron 5 genotype was similar between two groups. SR-BⅠ exon 1 allelic frequencies of G and A were 0.988, 0.012 and 0.997, 0.003 in CHD and control groups respectively. There was no significant difference between the distribution of AluI polymorphism and the allele frequency in CHD and control groups （P>0.05）. Serum levels of high density lipoprotein cholesterol （HDLC） and apolipoprotein AⅠ （ApoAⅠ） in the GA＋AA genotype subgroup was significantly higher than in GG genotype subgroup among CHD group male subjects （P<0.05）. Conclusion The exon 1 polymorphism of SR-BⅠ gene may not be associated with susceptibility to CHD and severity degrees of coronary lesions in Tianjin Han Chinese population, but A allele of SR-BⅠ exon 1 can lead to the elevated HDLC and ApoAⅠ concentration in male CHD patients.