Aim To explore effect of compound Danshen dripping pill on apoptosis of cardiac muscle cells and apoptosis-related gene expression in rats with acute myocardial infarction. Methods 45 healthy majority SD rats were randomly divided into sham operation group, acute myocardial infarction group and compound Danshen dripping pill group. Rats in sham operation group underwent thoracotomy without coronary ligation. Rats in acute myocardial infarction group and compound Danshen dripping pill group underwent with coronary ligation of left anterior descending branch to establish AMI rats model. Rats in sham operation group and acute myocardial infarction group were given physiological saline. Rats in compound Danshen dripping pill group were given compound Danshen dripping pill . After 3 days，killing the rats and isolating the cardiac tissue . Fas，Fas-L and p53 mRNA of cardiac tissue in three group were analyzed by real time PCR. Bcl-2 and Bax in three groups were analyzed by western blot. Apoptosis cells index of cardiac cell in three groups were analyzed by TUNEL and Western blot. Results The mRNA of Fas，Fas-L and p53 in acute myocardial infarction group and compound Danshen dripping pill group were significantly higher than that in sham operation group （P<0.05）. The mRNA of Fas， in compound Danshen dripping pill group was significantly lower than that in acute myocardial in farction group （P<0.05）. Bcl-2/Bax ratio in acute myocardial infarction group was significantly lower than that in sham operation group （P<0.05）, but there was no statistical difference between sham operation and compound Danshen dripping pill group （P>0.05）. Apoptosis cells index of cardiac cell in acute myocardial infarction group and compound Danshen dripping pill groups significantly higher than that in sham operation group （P<0.05）. Apoptosis cells index of cardiac cell in compound Danshen dripping pill groups significantly lower than that in acute myocardial infarction group （P<0.05）. Conclusion Compound Danshen dripping pill can reduce apoptosis-related gene expression in rats with acute myocardial infarction and inhibit apoptosis of cardiac cell.