Testosterone Enhances the Proliferation of Peripheral-Blood- Derived Endothelial Progenitor Cells by up-regulating Vascular Endothelial Growth Factor Expression
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    Abstract:

    Aim To explore the effects and related mechanisms of testosterone on the proliferation of Peripheral-blood endothelial progenitor cells (PB-EPCs). Methods Total mononuclear cells(MNC) were isolated from peripheral blood of healthy volunteers by Ficoll density gradient centrifugation, culturing with EGM-2MV for 7 days in vitro. The adherent cells showed up taking of acetylated low-density (ac-LDL-DiI) and binding of lectin(FITC-UEA-I), observing with confocal laser scanning microscopy. PB-EPC were dealt with four concentrations of testosterone, as 0 nmol/L, 1 nmol/L, 10 nmol/L,and 100 nmol/L respectively, and in another group PB-EPC were pretreated with 10 nmol/L flutamide (androgen receptor antagonist) for 3h and then stimulated with 10 nmol/L testosterone. After 48 h, the ability of cell proliferation was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diph-phenyltetrazolium bromide assay (MTT). The VEGF expression was tested by quantitative real-time RT-PCR and ELISA assay. Results The proliferation of PB-EPC were increased by testosterone in a dose-dependent manner, however, these effects could be blocked by flutamide. Testosterone can up-regulate VEGF both on mRNA and protein level, however, these effects could be blocked by flutamide. Conclusions Testosterone enhances the proliferation of PB-EPCs by up-regulating VEGF expression via androgen receptor pathway.

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XUE Ya-Wei, REN Gong-Qing, WANG Zhi, SUN Wen-Wen,,ZHANG Hao. Testosterone Enhances the Proliferation of Peripheral-Blood- Derived Endothelial Progenitor Cells by up-regulating Vascular Endothelial Growth Factor Expression[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2013,21(03):225-228.

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  • Received:April 06,2011
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