Effect of Atorvastatin on Vascular Endothelial Activation Induced by HMGB1
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    Abstract:

    Aim To explore whether atorvastatin inhibits HMGB1-induced vascular endothelial activation, and clarify the underlying molecular mechanism. Methods The cultured endothelial cells (EC) of rats were treated by Atorvastatin, HMGB1,TLR4-specific inhibitor CLI-095 with different concentrations. Leukocyte-endothelial adhesion was calculated as the proportion of the adherent neutrophils fluorescence intensity among the total added cells. RT-PCR and Western blot method were used to assay the expression of Toll like receptor 4(TLR4), ICAM-1 , E-selectin mRNA and protein. DNA binding activity of NF-κB was measured by EMSA. Results Atorvastatin, at concentrations ranging from 0.1 to 10 μmol/L, effectively and in a dose-dependent manner inhibited HMGB1-induced ECs activation. Atorvastatin markedly suppressed TLR4 expression (P<0.05) in ECsIncubation of ECs with atorvastatin and CLI-095 reduced HMGB1-induced NF-κB p65 DNA binding activity and adhesion molecules (ICAM-1 and E-selectin) expression (P<0.05). Conclusion Atorvastatin can attenuate HMGB1-induced vascular endothelial activation. The underlying mechanism may be connected with inhibition of TLR4/NF-κB-dependent signaling pathway induced leukocyte-endothelial adhesion.

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LI Xin-Xin, YANG Jun, YANG Jian, DING Jia-Wang, LI Song,,DONG Wu-Song. Effect of Atorvastatin on Vascular Endothelial Activation Induced by HMGB1[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2013,21(03):193-197.

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  • Received:April 06,2012
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