Aim To know means and distribution of cholesterol synthesis and absorption markers in healthy persons. Methods 64 healthy adults were selected in medical examination center of Anzhen hospital and conventional determination of liver and kidney function,serum total cholesterol （TC）,high density lipoprotein cholesterol （HDLC）,triglyceride （TG）,fasting plasma glucose （FPG）. Serum markers of cholesterol synthesis and absorption were determined with gas chromatogram. Results The sum of the average of five kinds of non-cholesterol steroids was 1.02 mg/dL,approximately 0.72% of total cholesterol,and there were individual differences. Serum lathosterol level in male was significantly higher than women （P<0.05）,while serum stigmasterol in male were significantly lower than women （P<0.01）. Synthesis markers of squalene decreased with the age increasing and absorption markers of stigmasterol increased with the age increasing. Conclusions It is through the detection of cholesterol absorption and synthesis markers to learn the distribution of cholesterol metabolism markers in healthy individuals.