Aim To study the effects of Echinocystic acid on the protein expression of apoptosis-associated Bcl-2,Bax and PARP （89 kDa） in the primary cultured rat cardiomyocytes subjected to anoxia/reoxygenation injury. Methods The cardiomyocytes were divided into normal group,anoxia/reoxygenation injury group,anoxia/reoxygenation preconditioning group,different concentration Echinocystic acid groups （0.5 μmol/L,5 μmol/L and 50 μmol/L）. Cardiomyocytes were subjected to anoxia for 3 hours and subsequently reoxygenation for 2 hours. Cell viability and LDH activity in medium were measured. The expression o f Bcl-2 and the apoptotic protein Bax and PARP （89 kDa） were detected by Western Blot. Results Compared with that of the control group,the expression of Bcl-2 protein in cardiomyocytes decreased significantly （P<0.01） and the expression of Bax protein and PARP （89 kDa） in cardiomyocytes increased significantly （P<0.01） after reoxygenation. Cell viability decreased obviously （P<0.05）. Compared with that of the anoxia/reoxygenation group,pretreatment with different concentration Echinocystic acid decreased LDH activity and increased cell viability（P<0.05）,the expression of Bcl-2 protein in the Echinocystic acid （5 μmol/L） groups increased significantly （P<0.05） and the expression of Bax protein decreased significantly （P<0.05）. Conclusion The antiapopotic effects of Echinocystic acid might be attributed to the up-regulated expression of Bcl-2 gene and the inhibited expression of Bax and PARP（89 kDa） gene expression.