Aim To evaluate the diagnostic accuracy of coronary artery stenosis with 320-slice dynamic volume computed tomography （320-DVCT） in comparison of selective coronary angiography. Methods Eighty-five patients with suspected coronary artery disease underwent 320-DVCT coronary angiography and selective coronary angiography subsequently. All available coronary segments were included in the analysis, regardless of size or image quality. Lesion with ＞50% diameter stenosis were considered significant. Image quality was analyzed, and the sensitivity, specificity, positive and negative predictive value of mild, moderate, severe coronary stenosis, occlusive coronary artery and ≥50% stenosis were calculated. Results The images acquired from 320-DVCT were excellent and sufficient and can be used to evaluate coronary stenosis. The sensitivity, specificity, positive and negative predictive value of 320-DVCT for detecting severe coronary stenosis, occlusive coronary artery and ≥50% stenosis were 90.9%, 99.2%, 89.6%, 99.3% and 98.6%, respectively, by mild stenosis, 92.8%, 98.9%, 90.9%, 99.2% and 98.3%, respectively, by moderate stenosis, 87.4%, 98.8%, 89.2%, 98.6% and 97.6%, respectively, by severe stenosis, 50.0%, 99.9%, 66.7%, 99.8% and 99.7%, respectively, by occlusive coronary artery, 94.1%, 97.2%, 89.2%, 98.5% and 96.6%, respectively, by ≥50% coronary artery stenosis. Conclusions 320-DVCT is a new, noninvasive approach of assessing the stenosis of coronary artery and can provide accurate and reliable information.