AimTo investigate the effect of platelet-derived growth factor-B (PDGF-B) expression derived from human umbilical vein endothelial cell-12 (HUVEC-12) on the cell migration of human umbilical arterial smooth muscle cells (HUASMC).MethodsHUVEC-12 cells were pretreated with different concentrations of amlodipine (0, 0.1, 10, 10.0 μmol/L) for 30 min and then incubated with 50 mg/L oxidized low density lipoprotein (ox-LDL) for 24 h.PDGF-B protein expression was detected by enzyme-linked immunosorbent assay (ELISA) in the culture medium.HUASMC were pretreated with 10.0 μmol/L amlodipine or 20 μmol/L PDGF-B antibody for 30 min respectively and then incubated with PDGF-B suspernatant fluid for 24 h, the cell proliferation was determined by thiazolyl blue (MTT) and the migration was determined by Transwell migration experiment.ResultsAmlodipine could down-regulate the PDGF-B protein expression in HUVEC-12 cells induced by ox-LDL.The cell proliferation and migration were both significantly decreased in the amlodipine pretreated group, the same as PDGF-B antibody pretreated group.ConclusionAmlodipine inhibits HUASMC proliferation and migration by down-regulating endothelial cell-derived PDGF-B expression.