Overexpression of MicroRNA29a Regulates Cells Apoptosis in Rat Cardiomyocytes
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    Abstract:

    AimTo investigate the role of microRNA-29a (miR-29a) on apoptosis in rat cardiomyocytes.MethodsIn our study, newborn rats cardiomyocytes was isolated, cells were transfected with miR-29a mimic by lipofectamine RNAiMAX, and then real time RT-PCR was used to measure the level of miR-29a, flow cytometry (FCM) was used to detect the cells apoptosis, and western blot was used to detect the protein expressive level of Caspase-3 and Caspase-9.ResultsMiR-29a mimics could upregulate the level of miR-29a in rat cardiomyocytes after 48h of transfecting, accompanied with the increase of cells apoptosis rate, and descent of the protein level of caspase-3 and caspase-9 in cardiomyocytes when compared to the control group(P<0.05).ConclusionOur study demonstrated that the overexpression of miR-29a may regulate cells apoptosis of rat cardiomyocytes, and the mechanisms among it may be concerned with Caspase-3 and Caspase-9 pathway.

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ZHANG ZhenHui, LI Jiao, LIU BenRong, LI AiQun,,LIU ShiMing. Overexpression of MicroRNA29a Regulates Cells Apoptosis in Rat Cardiomyocytes[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2012,20(12):1079-1082.

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  • Received:July 16,2012
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