Effect of Adipocyte Fatty Acid Binding Protein Silencing on Triglyceride Production and Visfatin Secretion in 3T3-L1 Adipocyte
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    Abstract:

    Aim To explore the influence of adipocyte's triglyceride anabolism and visfatin secretion after silence adipocyte fatty acid binding protein(A-FABP) genes by RNA interference. Methods 3T3-L1 preadipocytes were cultured in vitro and were induced to differentiate into adipocytes,then the adipocytes were cultured with concentration from 0 to 1 mmol/L fatty acid,adipocyte's triglyceride production and visfatin secretion were measured.The micro RNA(miRNA) expression vector plasmid about A-FABP genes was constructed and then transfected with the 3T3-L1 mature adipocytes.A-FABP mRNA expression and A-FABP protein expression were measured by reverse transcription polymerase chain reaction(RT-PCR) and Western blot.After and before slience A-FABP,the adipocytes were cultured with concentration of 0.5 mmol/L fatty acid,visfatin mRNA expression in adipocyte were measured by RT-PCR,adipocyte's triglyceride anabolism and visfatin secretion were detected. Results With the concentration of fatty acid risen in adipocytes,the concentration of triglyceride in adipocyte were raised(P<0.05),the concentration of visfatin in adipocyte were raised too(P<0.05).Constructing the miRNA expression vector plasmid about A-FABP genes,then transfecting the 3T3-L1 mature adipocytes,the expression of mRNA and protein about A-FABP genes were obviously restrained(P<0.05).After silence AFABP genes,the concentration of adipocyte's triglyceride was obviously lower than before(P<0.05),the concentration of visfatin secretion was obviously lower than before(P<0.05). Conclusion After silence A-FABP genes by RNA interference,the concentration of adipocyte's triglyceride anabolism and visfatin secretion were lower than before.

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WU Jie, ZOU Jin, and ZHONG Min. Effect of Adipocyte Fatty Acid Binding Protein Silencing on Triglyceride Production and Visfatin Secretion in 3T3-L1 Adipocyte[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2011,19(12):973-978.

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  • Received:July 05,2011
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