Aim To investigate the effects of deuterium-depleted water(DDW) and liquor on lipid metabolism,blood coagulation and fibrinolysis activity in experimental hyperlipidemic rats. Methods Normal control and hyperlipidemia model groups were designed.Experimental hyperlipidemia rats were administration of liquor [(0.01 L/(kg·d)] or deuterium-depleted water for 90 d.The levels of plasma triglyceride(TG),total cholesterol(TC),high-density lipoprotein(HDL),and low-density lipoprotein(LDL)were determined,and prothrombin time(PT),activated partial thromboplastin time(APTT),tissue plasminogen activator(t-PA)activity,and plasminogen activator inhibitor-l(PAI-1) activity were measured.The pathological examination of the aorta structure and liver tissue was performed. Results Compared with model group,HDL and t-PA significantly increased(P<0.05) and PAI-1 significantly decreased(P<0.05)in DDW group. LDL and PAI-1 significantly decreased(P<0.05) and PT and t-PA significantly increased(P<0.05) in low-dose liquor group.Further,TC,HDL,and PT and t-PA in high-dose liquor group significantly increased(P<0.05).The TC,HDL,LDL,and PT and t-PA significantly increased(P<0.05) and TG and PAI-1 significantly decreased(P<0.05) in high-dose liquor with DDW group.The TC,TG,and t-PA significantly increased(P<0.05) and APTT and PAI-1 significantly decreased(P<0.05) in low-dose liquor with DDW group. Conclusions Blood clotting system and fibrinolysis system in the experimental hyperlipidemic rats were improved by DDW and liquor,respectively.The combined effects of liquor and DDW on these 2 systems in hyperlipidemic rats were similar to those of liquor.