Effects of Tetrahydroxystilbene-2-O-β-D-Glucoside on the Expression of Bcl-2 and Bax in the Apoptosis of HUVEC induced by H_2O_2
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    Abstract:

    Aim To investigate the effects of tetrahydroxystilbene-2-O-β-D-glucoside(TSG) on the apoptosis of human umbilical vein endothelial cells(HUVEC) and its molecular mechanisms induced by hydrogen peroxide(H2O2).Methods The model of apoptosis HUVEC was induced by 300 μmol/L H2O2,MTT assay was used to detect the cell proliferation,flowing cytometry was used to measure the effect of apoptosis.The expression of Bcl-2 and Bax was detected by Western blot.Results H2O2 could inhibit the viability of cells,decrease the expression of Bcl-2 and the ratio of Bcl-2/Bax,and increase the apoptosis ratio and the expression of Bax.On the contrary,treatment of TSG could improve the rate of cell proliferation,inhibit cell apoptosis,up-regulate the expression of Bcl-2 and increase the ratio of Bcl-2/Bax,down-regulate the expression of Bax significantly(P<0.01).Conclusions TSG could inhibit H2O2-induced apoptosis of HUVEC,and the mechanisms might be associated with regulating the expression of Bcl-2,Bax and the ratio of Bcl-2/Bax.

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LONG Shi-Yin, HUANG Liang-Zhu, QIAO Xin-Hui, GAO Xi-Qiang, DING Lan, ZHANG Cai-Ping, CHEN Wu-Zhe, and TIAN Ying. Effects of Tetrahydroxystilbene-2-O-β-D-Glucoside on the Expression of Bcl-2 and Bax in the Apoptosis of HUVEC induced by H_2O_2[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2011,19(7):557-560.

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  • Received:May 17,2011
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