Aim To explore the mechanisms of microRNA 200a in hepatic insulin resistance induced by interleukin-6(IL-6),specifically to in-vestigate the role of microRNA 200a in hepatic glucogen and insulin signal pathway transduction.Methods The cell model forhepatic insulin resistance was induced by IL-6(10μg/L) for 24 hours in NCTC1469 cell.The level of glucogen was reduced.The expression of microRNA 200a was tested by q-PCR.The level of glucogen was quantified in NCTC 1469 transfected with microRNA200a mimics.The target gene of microRNA200a was predicted by bio-information software.The protein level of the target gene wastested by Western Blotting.Results The level of glucogen and the insulin signal pathway was inhibited in NCTC 1469 cells trea-ted by IL-6.The expression of microRNA 200a was decreased in NCTC 1469 cell induced by IL-6.The level of glucogen and theinsulin signal pathway were stimulated in NCTC 1469 cell transfected by microRNA 200a mimics.The protein level of Pten was de-creased by overexpression of microRNA200a.Conclusions IL-6 downregulates the expression of microRNA200a in hepatic insu-lin resistant NCTC 1469 cells.The microRNA 200a stimulates biosynthesis of glucogen as well as insulin signal pathway accompanied by down-regulating the expression of Pten.