AimTo investigate the effect of ODN1826, a TLR9 ligand, on perilipin 2 expression in macrophages. Methods Real-time PCR and Western blotting were performed to detect perilipin 2 expression.Transient transfection and luciferase assay were employed to measure the perilipin 2 promoter activity. ResultsODN1826 significantly induced perilipin 2 expression in a dose and time dependent manner in macrophages.ODN1826 markedly enhanced perilipin 2 promoter activity, and this process requires Ets/AP-1 activity. Conclusions TLR9 signal pathway stimulated perilipin 2 expression in macrophages, in addition, ODN1826-induced perilipin 2 promoter activity requires Ets/AP-1 activity.