AimTo investigate the effects of hydrogen sulfide(H₂S) on ox-LDL uptake of THP-1-derived macrophages and its underlying mechanisms. Methods Fluorescence microscopy, RT-PCR and western blot were performed. ResultsIncubation of macrophages with DiI-ox-LDL led to ox-LDL uptake of macrophages.Sodium hydrosulfide(NaHS, an H₂S donor) decreased ox-LDL uptake. While DL-propargylglgycine (PPG) exerted opposite effects.Furthermore, ox-LDL markedly induced CD36 mRNA and protein expression in macrophages, which was abolished by NaHS (50 to 100 μmol/L), but enhanced by PPG. Conclusions H₂S inhibits ox-LDL uptake of THP-1-derived macrophages and down-regulates CD36 mRNA and protein expression.