Aim To establish the model of murine macrophage RAW264.7 derived foam cell and identify them with simple and accurate method. Methods The experiment was devided into normal control group and six experimental groups of different concentration of oxidized low density lipoprotein(ox-LDL) incubated with cell.On the incubation time for 24 hours that MTT method and flow cytometry(FCM) were used to determine the suitable range of ox-LDL concentration,then measure intra-cellular cholesterol ester in different level of macrophage foam cell with total cholesterol and free cholesterol kit. Results Cell viability had been significantly inhibited when ox-LDL concentration was at the range of 20～30 mg/L,when ox-LDL concentration was greater than 40 mg/L,apoptosis cells continuously necrosis.Ox-LDL at the concentration of 20 and 30 mg/L,incubated with cell for 24 hours,the propotion of intra-cellular cholesterol ester were 66.26% and 71.19%.Foam cell induced by 10 mg/L of ox-LDL was not typical,and by 40 mg/L of ox-LDL or more,severe degree of foam cell led to cells floating,most cells were rupture or apoptosis,lipid droplet dispersed in extra-cellular. Conclusion Ox-LDL at the concentration range of 20～30 mg/L,incubated with RAW264.7 for 24 h,induced foam cell has good morphology,and model stability,meet the morphology feature of foam cell.