Aim To explore the clinical value of the content of Angiopioetin-like protein 3(ANGPTL3)in patients with hyperlipoidemia by ELISA. Methods Serum ANGPTL3 was examined by ELISA in the patients with hypercholesterolemia(HTC),hypertriglyceridemia(HTG) and patients with both of them,and compared with normal group. Results In comparison with the normal group,the content of serum ANGPTL3 of patients with HTG was increased significantly(P>0,05),and there was no significant difference in diagnosis of HTG between detecting ANGPTL3 by ELISA and TG by GOD-POD method.But the content of serum ANGPTL3 of patients with HTC made no difference with normal group(P>0.05).And variance in the contents of serum ANGPTL3 of patients with both of HTC and HTG was not significant. Conclusion ELISA method can detect the increase in the patients with HTG,and there was no difference in the patients with HTC sensitively,which laid a foundation for application of ANGPTL3 ELISA kit in clinical laboratory.