Aim To investigate the contribution of three renin angiotensin system(RAS)gene polymorphisms to the presence of peripheral vascular disease(PVD)in Chinese type 2 diabetes(T2DM)patients.Methods Amount to 385 consecutive T2DM patients were examined.Fifty-six of these were determined as PVD patients for their ankle brachial index(ABI)were less than 0.9.84 non-PVD individuals were enrolled as control group.The clinic parameters and the gene polymorphisms of angiotensin converting enzyme(ACE)I/D,angiotensin Ⅱ type 1 receptor(AT1R)A1166C and angiotensinogen(AGT)M235T were measured.Logistic analysis were used to study the relationship between the parameters and the risk of PVD.Results There were differences in fibrinogen(Fbg),serum creatinine(Scr)and high-sensitivity C-reactive protein(hs-CRP)between cases and controls.There were no significant differences in the genotypes and allele frequencies of the three RAS gene polymorphisms between two groups.However,in the T2DM patients with D allele of ACE I/D,the prevalence of PVD significantly increased when they had T allele of AGT M235T at the same time.Logistic regression analysis showed that Fbg,hs-CRP,and AGT M235T were the risk factors of PVD.Conclusion Inflammation factors such as hs-CRP and Fbg are involved in the development of PVD in the T2DM groups.Although the role of single gene polymorphism of RAS is limited,the interaction of the three RAS gene polymorphisms may have significant effect on the risk of PVD in T2DM patients.The individuals with T2DM presenting with D allele of ACE I/D are likely to have higher risk of PVD when they have T allele of AGT M235T at the same time.