AimTo approach polymorphism distribution of angiotensin converting enzyme （ACE） and cytochrome P450 （CYP） 3A5 gene of hypertensive patients in Hunan province.MethodsThere were 583 hypertensive patients.ACE gene polymorphisms of 403 hypertensive patients in Loudi and Xiangtan were investigated.While, CYP3A5 gene polymorphisms of 180 hypertensive patients were investigated.ResultsACE allele frequency was I (55.8%) and D (44.2%), in order with ID as the highest genotype.CYP3A5 allele frequency was *1 (35.0%) and *3 (65.0%), in order with *1*3 as the highest genotype.No sex difference was found in genotypes and allele frequency distributions (all P>0.05).ConclusionPolymorphism distribution of ACE and CYP3A5 gene of hypertensive patients in Hunan province reached Hardy-Weinberg genetic balance, hypertension related drug metabolism enzyme and receptor gene polymorphisms showed the individual differences, which provided the previous genetical evidences for single-drug therapy in hypertension population.