Effects of Diallyl Trisulfide Coating Stent on the Expression of Metalloproteinase-2 and Tissue Inhibitor of Matrix Metalloproteinase-2 in a Canine Model of Coronary Restenosis
Author:
  • Article
  • | |
  • Metrics
  • | | | | |
  • Comments
    Abstract:

    AimTo investigate the effect of diallyl trisulfide (DT)-coated coronary stents on expression of metalloproteinase-2 (MMP-2) and tissue inhibitor of matrix metalloproteinase-2 (TIMP-2) in canine model of restenosis.MethodsDT-coated stents (210 μg/stent) were deployed with mild oversizing in the left circumflex coronary arteries (LCX) or the left anterior descending coronary arteries (LAD)of 14 canines.The implantation of protein-coated stents were used as control.Histopathologic examination was performed 28th days after stents implantation.Total RNA was isolated from the arterial wall at different time (5 h, 1 d, 7 d, 14 d and 28 d) after stents implantation.RT-PCR method was adopted for detection of MMP-2 and TIMP-2 genes expression.ResultsNeointimal hyperplasia and the restenosis rate were less in DT-coated stents than those in control group (P<0.01).MMP-2 was constitutively expressed in uninjured coronary arteries, and its activity did not increase until 7 days and remained elevated up to 28 days after the implantation of protein-coated stents.MMP-2 genes expression in DT group was reduced at 5 h, 1 d, 14 d and 28 d compared with that in control groups (P<0.01).The expression of TIMP-2 genes was not significantly different at 5 h, 1 d and 14 d after the implantation of DT-coated stents and protein-coated stents.At 7 d and 28 d ,TIMP-2 genes expression increased in DT group compared with that in control groups (P<0.05).ConclusionDT-coated coronary stents can decrease the expression of MMP-2 and enhance the expression of TIMP-2 in the canine LAD or LCX after implantation, which could therefore contribute to decreasing the effects of its neointimal hyperplasia.

    Reference
    Related
    Cited by
    Comments
    Comments
    分享到微博
    Submit
Get Citation

XU Shang-Hua, LUO Shun-Xiang, ZHONG Wen-Liang,,OU Mei-Yuan. Effects of Diallyl Trisulfide Coating Stent on the Expression of Metalloproteinase-2 and Tissue Inhibitor of Matrix Metalloproteinase-2 in a Canine Model of Coronary Restenosis[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2010,18(2):129-133.

Copy
Share
Article Metrics
  • Abstract:1035
  • PDF: 1082
  • HTML: 0
  • Cited by: 0
History
  • Received:December 15,2009
  • Revised:February 10,2010
Article QR Code