The Expression Development of Proapoptic Molecule C/EBP Homology Protein mRNA and Protein Following Focal Cerebral schemia/Reperfusion in Rats
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    Abstract:

    AimTo detect the expression development of proapoptic molecule C/EBP homology protein(CHOP) mRNA and protein, and explore its effect on neuronal apoptosis after cerebral ischemia/reperfusion in rats.MethodsTransient focal cerebral ischemia was induced by middle cerebral artery occlusion (MCAO) for 2 hours followed by reperfusion in sprague-dawley rats.Then,the expression of CHOP protein and/or mRNA were measured with methods of immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) at 1 h, 3 h, 6 h, 12 h and 24 h after reperfusion in cerebral cortex of rats.The neuronal apoptosis was detected by the method of terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL).ResultsIn cerebral ischemia model group,the expression level of CHOP mRNA reached a peak at 12 h after reperfusion and that of CHOP protein reached a peak at 24 h after reperfusion,which paralleled with the tendency of neuronal apoptosis development.ConclusionCerebral ischemia reperfusion may induce CHOP expression. CHOP may play an important role in neuronal apoptosis induced by cerebral ischemia/reperfusion.

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SHEN Xiang-Min, YANG Qi-Dong, TAN Li-Ming, LIU Yun-Hai, TANG Zhen-Yu, HUANG Qing,,ZHOU Lin. The Expression Development of Proapoptic Molecule C/EBP Homology Protein mRNA and Protein Following Focal Cerebral schemia/Reperfusion in Rats[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2010,18(1):43-46.

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History
  • Received:September 28,2009
  • Revised:January 05,2010
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