AimTo investigate the effects of 7-difluoromethyl-5,4’-dimethoxygenistein (dFMGEN) on the adhesion of monocytes to H2O2-induced endothelial cells, and its molecular mechanism.MethodsThe effects of dFMGEN on the adhesion rate of monocytes and endothelial cells were examined by measuring protein content.The expression of E-selectin and intercellular adhesion molecule-1 (ICAM-1) in H2O2-induced endothelial cells were measured by enzyme-linked immunosorbant assay (ELISA).The effects of dFMGEN on the expression of nuclear factor-κB (NF-κB) and phosphorylated extracellular signal-regulated kinase 1/2 (p-ERK1/2) were determined using Western Blotting.ResultsWhen endothelial cells were treated with 1.0 mmol/L H2O2 for 24 h, the adhesion rate was added, the release of E-selectin and ICAM-1 were increased, and the expression of NF-κB and p-ERK1/2 were up-regulated. After pretreating with dFMGEN, results showed that dFMGEN can inhibit the adhesion between monocytes and endothelial cells induced by H2O2, reduce the release of E-selectin and ICAM-1, and down-regulate the expression of NF-κB and p-ERK1/2.ConclusiondFMGEN effectually inhibits the adhesion between monocytes and endothelial cells induced by H2O2, which is probably due to the inhibition of the expression of E-selectin and ICAM-1 and downregulation of NF-κB and p-ERK1/2.