Aim To package heat shock protein 72 recombinant adenoviruses and to explore its effect on ECV304 cell apoptosis.Methods Heat shock protein 72 recombinant adenoviruses was generated by employing homologous recombination in bacteria,flow cytometric analysis was used to invest the effect of heat shock protein 72 recombinant adenoviruses on cell apoptosis,luciferase reporter system and western blotting were used to analyse its effects on p53 Transcription and expression.Results Heat shock protein 72 recombinant adenoviruses were successfully packaged,and heat shock protein 72 could enhance cell apoptosis.Conclusions Heat shock protein 72 may contribute to cell apoptosis by upregulating P53 expression.