Aim To explore the dynamic change of antibobies in rats,mice and rabbits when they were treated with apolipoprotein A1-P11(ApoA1-P11) and the reactionogenicity of antibodies were compared among them,then to determine the suitable animal which can be repeately treated with ApoA1-P11 for long time.Methods 14 rats,14 mice and 14 rabbits were each randomly divided into two groups:the ApoA1-P11 group and the control group.The ApoA1-P11 group received ApoA1-P11(1 mg/kg weight,once every other day) respectively,and the control group received normal saline in the same way.The serums of both groups were collected before the administration,in the week 1,2,3 and 4 after the last injection.The chang pattern and titers of antibodies were determined by ELISA.Results The rising and dropping patterns of antibodies in rats,mice,and rabbits were similar,but the level of antibodies produced by rabbits was much higher than that of rats and mice.Conclusions The rats and mice are more suitable for evaluation of ApoA1-P11's effects for long-time use,to whom correspondance should be addressing compared with rabbits,which laid a foundtion for ApoA1-P11 used for treating hyperlipemia and atherosclerosis.