The Impact of Ischemia-Preconditioning on Expression of Hypoxia Inducible Factor-1α Gene in Acute Myocardial Infarction Rats and the Involvement of PKC Signal Pathway
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    Abstract:

    Aim To study the expression of hypoxia-inducible factor-1α(HIF-1α) in myocardial infarction rats and the role of protein kinase C (PKC) in signal pathway of hypoxia-inducible factor-1α gene expression. Methods 32 healthy male Wistar rats were divided into four groups:ischemic preconditioning group,simple myocardial infarction group,ischemic preconditioning plus protein kinase C inhibitor group and sham-operation group. Rats in treated groups received ligation of the proximal left anterior descending coronary artery to induce acute myocardial infarction. The sham-operation group dont take ligation of coronary artery. In ischemic preconditioning plus PKC inhibiter group,rats were injected with the protein kinase C inhibitor (chelerythrine,5 mg/kg,iv). After 1 day of operation,rats were killed. Results The infarct size in ischemic preconditioning group decreased significantly than that in ischemic preconditioning plus protein kinase C inhibitor group and simple myocardial infarction group (P<0.01). The hypoxia-inducible factor-1α mRNA gene expression increased in ischemic preconditioning group more than that in ischemic preconditioning plus protein kinase C inhibitor group and simple myocardial infarction group. Hypoxia-inducible factor-1α protein expression had the same change. Conclusions Hypoxia-inducible factor-1α play aprotective role in ischemic preconditioning; protein kinase C is the important signal pathway of hypoxia-inducible factor-1α gene expression.

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NIU Tie-Sheng, QI Guo-Xian, FU Peng, and SUN Ying-Xian. The Impact of Ischemia-Preconditioning on Expression of Hypoxia Inducible Factor-1α Gene in Acute Myocardial Infarction Rats and the Involvement of PKC Signal Pathway[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2008,16(10):796-800.

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History
  • Received:September 03,2008
  • Revised:October 18,2008
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