Effect of Stromal Interaction Molecule 1 Knockdown on Cell Cycle of Vascular Smooth Muscle Cell
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    Abstract:

    Aim To explore the effect of STIM1 knockdown on cell cycle of vascular smooth muscle cell. Methods Rat VSMC was isolated and primary cultured. Ad-si/rSTIM1 and Ad-hSTIM1 were transfected into VSMC. The protein of STIM1, p21 and pRb were measured by western blot, the cell cycle of VSMC was analyzed by flow cytometry. Results On 48 h after transfected Ad-si/rSTIM1, the expression of STIM1 protein was decreased significantly compared to that in Ad-hSTIM1-treated group (P<0.05). The percent of G0/G1 phase cell was significantly increased (P<0.05). The expression of p21 protein was increased and the expression of pRb protein was decreased (P<0.05). However, the cotransfection of Ad-hSTIM1 with Ad-si/rSTIM1 restored these responses. Conclusions STIM1 knockdown up-regulates the expression of p21 and down-regulates the expression of pRb, which inhibits cell to progress into S phase. Stim1 maybe have a key role in the vascular smooth muscle cell proliferation.

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GUO Rui-Wei, HUANG Lan, SONG Ming-Bao, WANG Hong,,GAO Pan. Effect of Stromal Interaction Molecule 1 Knockdown on Cell Cycle of Vascular Smooth Muscle Cell[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2008,16(9):701-703.

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History
  • Received:July 01,2008
  • Revised:September 21,2008
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