Aim To investigate the effect of advanced glycation end products (AGE) on number of endothelial progenitor cells (EPC). Methods AGE was prepared by incubating D-glucose and human serum albumin (HSA) for 90 days, and then identified by fluorescence and SDS-PAGE.The mononuclear cells (MNCs) were isolated from peripheral blood of healthy adults,then were plated on fibronectin-coated culture 6-well plates respectively in EC basal medium-2.After cultured 7 days, attached cells were identified with antibodies recognizing human CD34,CD133 and KDR, several groups of attached cells plated in the absence or presence of AGE (2 mg/L, 20 mg/L, and 200 mg/L) for 0 h, 24 h, 48 h, and 72 h respectively,the cells were quantified by viewing 10 random microscopic fields. Results AGE showed the absorption and fluorescent spectra. After cultured 7 days, attached cells were identified with antibodies, the results demonstrated the positive expression of antibodies were KDR (78.60%±2.20%), CD34 (8.60%±2.00%) and CD133 (2.80%±0.60%). The decrease number of EPC per field was observed under 200 mg/L AGE at 24 h (146.67±4.98), while reached to the minimum (73.67±3.76) at 72 h. However, no statistical change in cell number was identified when cells were treated under low density AGE (2 mg/L and 20 mg/L). Conclusion High AGE concentration may inhibit EPC augmentation.