Construction and Expression of Eukaryotic Expression Vector Containing Gene Cystatin C
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    Abstract:

    Aim To clone human Cystatin C gene and construct its eukaryotic expression vector to observe the expression in the human vascular smooth muscle cells (VSMC). Methods Cystatin C gene was obtained from human endothelial cells by reverse transcription-polymerase chain reaction (RT-PCR). Molecular cloning technique was used to construct this kind of eukaryotic expression vector. pCDNA3.1-Cystatin C was identified by restriction enzyme and PCR. Using liposome-mediated transfection, the eukaryotic expression vector pCDNA3.1-Cystatin C was transfected into human VSMC and proved by RT-PCR and Western blot. Results The eukaryotic expression vector pCDNA3.1-Cystatin C was constructed correctly and was highly expressed in human VSMC. Conclusion Cystatin C was cloned successfully, its eukaryotic expression vector was constructed correctly and was expressed highly in human VSMC.

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WANG Qun, LU Qing-Hua, JIANG Wei-Dong, DU Yi-Meng, WANG Xin, HAO Lin, DONG Zhao-Qiang, XU Dong-Ling,,SHENG Lin. Construction and Expression of Eukaryotic Expression Vector Containing Gene Cystatin C[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2008,16(2):97-100.

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History
  • Received:October 22,2007
  • Revised:January 08,2008
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