Aim To investigate expression of farnesoid X receptor(FXR) in murine macrophage cell line ANA-1 and evaluate the effect of FXR agonist chenodexycholic acid(CDCA)on the expression of monocyte chemoattractant protein-1(MCP-1) in ANA-1 cells.Methods ANA-1 cells were cultured and treated with FXR agonist CDCA.The mRNA level of FXR and MCP-1 were detected by reverse transcription-polymerase chain reaction(RT-PCR).Western blot was used to assess the expression of FXR and MCP-1 protein level.Results FXR expressed in ANA-1 cells and acted in an autoregulatory fashion.CDCA significantly reduced MCP-1 mRNA and protein level in ANA-1 cells(P<0.05).Conclusions FXR experss in murine macrophage cell line ANA-1.After activated by CDCA,FXR may downregulate MCP-1 directly or indirectly.