Aim To set up a method to measure the rate of reverse cholesterol transport from macrophage to feces in vivo,serum lipid profiles of mice was tested and the 3 H-contents was quantitated in serum,liver and feces in mice after 24 hours intraperitoneally were injected macrophages which were labeled with 3 H cholesterol.Methods 16 C57BL/6 mice were divided into two groups at random and one group was injected intraperitoneally with RAW264.7 macrophages which were loaded with cholesterol by incubation with acetylated low density lipoprotein(acLDL),labeled with 3 H-cholesterol.As a control,another group was injected with culture media instead.Serum lipid profiles were quantitated by enzymatic method;serum and liver tissues were harvested at 24 hours,feces were collected(0～24 h),and all were analyzed for tracer counts(all were expressed as the percent of the total injected counts per minute).Results After being injected intraperitoneally 24 h with DMEM or RAW264.7 macrophages,the serum lipid profiles of two groups have no difference(p> 0.05).3 H-cholesterol could be detected in the plasma,liver,and feces of the mice in tested group,and the percents of the total injected counts per minute were 2.810%± 0.060%,0.680% ±0.030% and 0.690%±0.030% respectively,also were higher than that of control group.Conclusions After being injected intraperitoneally with 3 H-cholesterol-labled RAW264.7 macrophages,the radioactivity could be detected in the plasma,liver,and feces of the mice.This method could be taken as a kind of feasible way to measure the rate of reverse cholesterol transport from macrophages into feces.