Aim To observe the myocardium damage caused by diabetic ketoacidosis with myocardial enzymogram and electrocardiogram and confirm their clinical significances. Methods 44 hospitalized patients had their vein blood drawn for determinating myocardial enzymogram which included creatine phosphokinase(CPK),creatinine phosphokinase-MB(CPK-MB),aspartate aminotransferases(AST) and cardiac troponin I(cTn-I);Meanwhile,the changes of electrocardiogram were recorded. The changes of myocardial enzymogram and electrocardiogram at acute stage were compared with those at catabatic stage. Results 44 patients with diabetic ketoacidosis had significantly higher levels of myocardial enzymogram at acute stage than those at catabatic stage(p<0.05).CPK-MB and CPK values of 44 patients at acute stage had no correlation(r=0.12,p>0.05).28(63.6%) patients with abnormal electrocardiogram changes at acute stage,and the major abnormities were 18(40.9%) sinus tachycardia,14(31.8%) ST-T change.The levels of myocardial enzymogram with abnormal electrocardiogram changes at acute stage were significantly higher than those with normal electrocardiogram changes(p<0.05). Conclusions The levels of myocardial enzymogram caused by diabetic ketoacidosis might be abnormally higher.There was transient and nonspecial damage to myocardium.The occurence of arrhythmia varied with the damage of diabetic ketoacidosis to myocardium.