Aim To detect the change of transcriptional profiling of C₂C₁₂ cells overexpressed krüppel-like factor 4(KLF4). Methods cDNA chip containing 14000 mouse cDNAs were employed to investigate the expression of genes influenced by KLF4 overexpression in C₂C₁₂ cells;bioinformatics database and software such as MEDLINE and Genomatix were performed to analyze the above genes. Results Two hundred and fifity genes were down-regulated in C₂C₁₂ cells overexpressed KLF4,with 155 known; Whereas 355 genes were up-regulated,with 255 known.Several genes about inflammatory or apoptosis were included. Conclusion Many downstream genes were influenced by KLF4 overexpression in C₂C₁₂ cells.