Aim To determine apolipoprotein AⅤ(ApoAⅤ)levels and study the correlation with lipid parameters in normolipidemic human subjects.Methods Lipids were measured enzymatically.Serum ApoAⅤ levels were determined using a novel,validated enzyme-linked immunosorbent assay.Results Serum ApoAⅤ level in normolipidemic subjects was rather low(5.4～455.6 μg/L).An inverse correlation between serum ApoAⅤ and triglyceride(TG)levels was observed(r=-0.225,P=0.031).In addition,ApoAⅤ concentration was positively related to high density lipoprotein cholesterol(HDLC)(r=0.453,p<0.001),and negatively related to body mass index(BMI)(r=-0.345,P=0.001).ApoAⅤ concentration was not related to low density lipoprotein cholesterol(LDLC)and total cholesterol(TC).Conclusions The serum ApoAⅤ level was rather low,and inversely related to TG and BMI,but positively related to HDLC.