Aim To observe the role of macrophage migration inhibitory factor(MIF) in proliferation of the human vascular endothelial cell line,EAhy926,cultured in vitro and the angiogenesis assay in vitro and vivo to prove that MIF could promote neovascularization. Methods Endothelial cell proliferation promoted by MIF was detected by MTT colorimetric assay.Angiogenesis in vitro was detected by ECMatrix gel,a numerical value was associated with a degree of angiogenesis progression.Angiogenesis in vivo was detected by the factorⅧ immunohistochemical staining,with injecting the matrigel plug in BALB/c Mice. Results MIF could promote the human endothelial cell proliferation,stimulate the tube formation of endothelial cells in vitro,and promote the b1ood vessels formation in matrigel plug in vivo. Conclusion Macrophage migration inhibitory factor plays a specific role in promoting neovascularization.