Aim To explore the cell apoptosis and inducible nitric oxide synthase(iNOS) expression in the cultured endothelial cells induced by tumor necrosis factor(TNF-α) and angiotensin Ⅱ(AngⅡ). Methods The cultured endothelial cells were treated with TNF-α(10 μg/L) and AngⅡ(1 μmol/L) in absence and presence of pyrroledithiocarbomate(PDTC);the mRNA of iNOS was measured by reverse transcriptionpolymerase chain reaction(RT-PCR),the protein of iNOS and IκBα were assessed by Western blotting,the activity of nuclear factor-κB(NF-κB) was evaluated by electrophoretic mobility shift assay(EMSA),and the cell apoptosis was detected with TUNEL. Ruselts The mRNA and protein of iNOS were significantly increased in the endothelial cells induced by TNF-α and AngⅡ(p<0.05) and PDTC could prevent this increase;at the same time,TNF-α and AngⅡ could increase the apoptosis of cells(p<0.05) and PDTC could prevent this increase. Conclusion TNF-α and AngⅡ induced the up-regulation of iNOS and the cell apoptosis,and the NF-κB play a key role in this process.