Aim To elucidate how advanced glycation end products(AGE) effected cyclooxygenase-2(COX-2) expression in cultured human umbilical vascular endothelial cells (ECV304). Methods ECV304 were cultured in vitro with AGE-human serum albumin(AGE-HSA).The levels of protein COX-2 were measured by Western blot. Results COX-2 expressed little in ECV304,AGE-HSA could induce COX2 expression,and the expression could be blocked by inhibiting the activation of NF-κB. Conclusion AGE-HSA could induce COX-2 expression by activing NF-κB.This pathobiological effect of AGEs might contribute to vascular lesion.