Aim To investigate the effects of atorvastatin on AngⅡ-induced hypertrophy myocytes and the changes of mRNA expression of peroxisome proliferators-activated receptorβ/δ in vitro. Methods Hypertrophy in neonatal rat cardiac myocytes(MC) was established with angiotensin Ⅱ(Ang Ⅱ) and treated with atorvastatin.The surface area of MC was analyzed by the aid of NIH Image J software,and the synthetic rate of protein in MC was detected by 3H-leucine incorporation.mRNA expression of atrial natriuretic peptide(ANP),brain natriuretic peptide(BNP) and peroxisome proliferatoractivated receptor beta/delta subtypes(PPARβ/δ) was measured by reverse transcription-polymerase chain reaction(RT-PCR). Results In the condition of hypertrophy,increases of surface area(p<0.01),mRNA expression of ANP,BNP(both p<0.01),and ~3H-leucine incorporation(p<0.01);and a decrease of mRNA expression of PPARβ/δ(p<0.01) in MC were detected,but no changes in treated with DMSO(p>0.05).Atorvatatin inhibited the changes above,reduced mRNA expression of ANP and BNP,elevated mRNA expression of PPARβ/δ in a dose-dependent manner(p<0.05). Conclusions It was suggested that atorvastatin has a potential role in the prevention and treatment of cardiac hypertrophy,and PPAR β/δ may be involved in it.