Aim To understand the association of fibrinogen and its gene β148, β854 polymorphism with coronary heart disease. Methods 1 254 subjects were divided into coronary heart disease group (coronary artery narrow≥50%, n=836) and control group (coronary artery narrow <50%, n=418) according to coronary angiographic results. TaqMan MGB probes (FAMTM and VICR dye-labeled) polymerase chain reaction (PCR) was performed to screen the fibrinogen gene β148, β854 polymorphism in the appropriate Sequence Detection System Instruments (7900HT). Plasma fibrinogen levels and blood lipids were simultaneously measured in the patients with coronary heart disease and controls. Plasma fibrinogen concentrations were assayed by thrombin method. Total cholesterol (TC), triglyceride (TG) and high density lipoprotein cholesterol (HDLC) levels were assayed by standard enzyme method. Results Age, sex, smoking, hypertension, diabete mellitus, blood lipids and fibrinogen levels in coronary heart disease group were very different from that of control group (p<0.01). No significant difference was found in the frequencies of fibrinogen β148, β854 alleles gene and genotype between the controls and the patients with coronary heart disease (p>0.05). There was no significant association between fibrinogen β148, β854 genotypes and fibrinogen and the patients with coronary heart disease (p>0.05), but age, sex, smoking, hypertension, diabete mellitus, TG, and HDLC, lipoprotein (a) and fibrinogen levels were closely associated with coronary heart disease (p<0.01). Conclusions Fibrinogen levels were closely associated with coronary heart disease, but not with fibrinogen β148, β854 genotypes.