Establishing the Expressive System of Human Low Density Lipoprotein Receptor Gene in the Oocyte of the Xenopus Laevis
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    Abstract:

    Aim The expressive system of low density lipoprotein(LDL) receptor gene was established in the oocyte of the Xenopus laevis and the system is applied to investigation of lowering-lipids and anti-atheriosclerosis. Methods The fluorescence on the membrane with DiI-LDL were determined by culturing the oocytes in the period V and VI so as to judge whether the endogenous LDL receptor in oocyte of xenopus laevis existed. After then the p3.7LDL (an expression plasmid which contain human LDL receptor gene) was transmit to the nucleus of oocyte and the immunofluorescence and immunity colloid golden in the cell membrane were observed under microscope. Results The experiments proved there was no endogenous LDL receptor on the membrane of oocytes in the period V and VI. The exogenous LDL receptor can be expressed after the p3.7LDL was transmitted to the nucleus of oocyte. Conclusion Human LDL receptor gene can be expressed in Oocyte of Xenopus laevis and the system can be used in the investigation of lowering-lipids and anti-atheriosclerosis.

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WO Xing-De, FAN Chun-Lei, LUO Yan, QIAN Ying, LU De-Zhao,,TANG Li-Hua. Establishing the Expressive System of Human Low Density Lipoprotein Receptor Gene in the Oocyte of the Xenopus Laevis[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2005,13(2):138-142.

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History
  • Received:May 08,2004
  • Revised:November 15,2004
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