Aim To investigate the relationship between G protein β3 subunit gene polymorphism and obesity in hypertensive patients. Methods Polymerase chain reaction combined with restriction enzyme digestion was used to detect the polymorphism of G protein of β3 subunit gene in 147 normotensive controls and 321 hypertensive patients. Body mass index was measured. Serum total cholesterol, triglyceride, high density lipoprotein cholesterol, low density lipoprotein cholesterol, fasting serum glucose were determined in hypertensive patients. Results There were no significant differences of the G protein β3 subunit gene C825T polymorphism genotypes and alleles between hypertensive patients (CC 28.7%, CT 52%, TT 19.3% and C 50.7%, T 49.3%) and normotensive controls (CC 27.2%, CT 46.9%, TT 25.9% and C 50.7%, T 49.3%). Body mass index, total cholesterol, triglyceride, high density lipoprotein cholesterol, low density lipoprotein cholesterol and fasting serum glucose did not differ significantly between CC and CT+TT genotype in hypertensive patients. Conclusion The C825T polymorphism of G protein β3 subunit was not associated with obesity in hypertensive patients.