Aim To investigate the effects of platelet-derived growth factor-BB (PDGF-BB)on content of calmodulin in human vascular smooth muscle cells. Methods The human vascular smooth muscle cells were cultured and effects of PDGF-BB on content of calmodulin in human vascular smooth muscle cells were assayed at different time and different concentration by its ability to stimulating calmodulin dependent cyclic nucleotide phsphodiesterase in vitro. Results Calmodulin level of quiescent human vascular smooth muscle cells was increased in its cell cycles with a maximal response 9 h, and PDGF-BB could significantly increase content of calmodulin of human vascular smooth muscle cells in a dose-dependent manner, with a maximal response at a concentration of 30 μg/L. Conclusion PDGF-BB could significantly increase calmodulin level of quiescent human vascular smooth muscle cells in a dose-dependent manner.