Effects of Tumor Necrosis Factor α on Plasminogen Activator Inhibitor-1 Expression and Regulation in Human Umbilical Vein Endothelial Cell
Author:
  • Article
  • | |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • |
  • Cited by
  • | |
  • Comments
    Abstract:

    Aim To study the influence of tumor necrosis factor α (TNF α) on plasminogen activator inhibitor 1 (PAI 1) activity, gene expression and regulation in human umbilical vein endothelial cell (hUVEC). Methods The PAI 1 activity in hUVEC culture medium was measured by chromogenic assay. The PAI 1 mRNA expression were determined by Northern blot. Using gene recombination techniques, four luciferase reporter gene plasmids containing different length of human PAI 1 gene promoter were constructed. Through the transient transfection analysis, the roles of AP 1 element (from -823 bp to -553 bp) in PAI 1 promoter have been determined. In order to further verify the role of AP 1 element, the three site directed mutants were received using polymerase chain reaction (PCR) and sequencing assay. Results The PAI 1 activity and mRNA level increased when hUVEC were exposed to 100 ku/L TNF α. At the same time, the AP 1 protein level increased in nuclear. The induction by TNF α decreased markedly when the three AP 1 elements in PAI 1 promoter have been mutated respectively. Conclusions TNF α could induce PAI 1 activity and mRNA in hUVEC; Increase of PAI 1 activity induced by TNF α was related to its mRNA expression; Three AP 1 elements in PAI 1 promoter may have an important role in PAI 1 gene transcriptions in endothelial cells induced by TNF α.

    Reference
    Related
    Cited by
Get Citation

LI Xiao Dong, ZHU Guang Jin, WANG Wen,, ZU Shu Yu. Effects of Tumor Necrosis Factor α on Plasminogen Activator Inhibitor-1 Expression and Regulation in Human Umbilical Vein Endothelial Cell[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2003,11(3):194-198.

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:October 18,2002
  • Revised:March 17,2003
Article QR Code