Aim To explore the effect of black rice outlayer fraction (BRF) on atherosclerotic plque formation in apolipoprotein E deficient mice and find the possible mechanisms of BRF in anti atherogenesis. Methods After 16 weeks intervention by 5% BRF, the atherosclerotic lesion area in aortic sinus were assessed by the method of image analysis and the serum anti ox LDL antibody levels were determined by ELISA. Results Serum anti ox LDL antibody levels in BRF group were lower than positive group and WRF group. This results correlated with the lesion area in aortic sinus. Conclusion BRF has dramatically reduced serum anti ox LDL antibody levels and this effect may be related to its anti atherogenesis.