Aim To get a rapid, simple label method for lipop rotein , an improved method was established with the fluorescent probe DiI. Method The serum at the bottom of the post ultracentrifuged tu be, instead of lipoprotein deficient serum (LPDS), was used as the medium to app lying in lipoprotein labeling. Results Binding assay showed that the labeled lipoprotein can bind with macrophage and chinese hamster ovocyte (CHO) cells with typical saturation able model. The results p roved that present method labeling lipoprotein have the normal lipoprotein ligan d binding properties. Conclusion This method is sim ple, rapid and reliable. It could be applied widely in lipoprotein receptor s tudy.