Aim To look for effective antioxidant to reduce risk of atherosclerosis. Methods The golden harmsters were fed for 10 weeks on a hypercholesterolemic diet. The animals received either vitamin C, vitamin E or juice of rose roxburghii tratt (JRRT) supplement in their diet except control animals. Results The level of corresponding antioxidants in various antioxidant-supplemented animals were increased compared to controls. The antioxidants induced the prolongation of lag time in low density lipoprotein (LDL) oxidation in vitamin C, vitamin E, JRRT groups compared with controls (221±56 min, 222±60 min, 248±48 min, and 181±47 min, compared between groups,P<0.05, respectively) and decreased the area of atherosclerosis lesion (2.63%±1.35%, 2.44%±1.47%, 1.43%±0.92%, and 5.62%±1.28%, compared between groups,P<0.001, respectively). Regression analysis showed that there was a negative relationship between the area of atherosclerosis lesion and LDL susceptibility. LDL susceptibilty was also correlated with the plasma level of vitamin E. Conclusions Antioxidants decrease extent of atherosclerosis lesion, and this may be caused by change in susceptibility of LDL oxidation. JRRT is a strong antioxidant for reducing risk of atherosclerosis.