Aim To explore the effects of different ratios of saturated, monounsaturated and polyunsaturated fatty acids of oral fatty loads on the postprandial lipids and lipoproteins metabolism. Methods The oral fatty loaded tests with three different fatty acids ratio. Results (1) Lipid concentrations: Triglyceride responded greatly to the oral fatty loads in three groups. The triglyceride in 2, 4, 6 hour plasma after the loads was significantly higher than fasting triglyceride, respectively (P<0.05). No significant total cholesterol changes occurred but high density lipoprotein cholesterol and low density lipoprotein cholesterol significantly decreased in group Ⅱ and Ⅲ. The increment of triglyceride at 2 hour after the test meal was significantly higher in group Ⅱ than group Ⅲ (P<0.05). Low density lipoprotein cholesterol in 6 hour plasma in group Ⅲ was significantly lower than that in group Ⅱ and Ⅲ (P<0.05). (2)Peak time:Triglyceride in group Ⅰ, Ⅱ and Ⅲ peaked at 2～4, 4 and 6 hours after the fatty load. High density lipoprotein cholesterol and apoprotein A1 in group Ⅱ and Ⅰ were lowest at 4 hours after the loads but at 6 h in group Ⅲ. (3)Area under the curve: Area under the curve of low density lipoprotein cholesterol in group Ⅲ was markedly smaller than in group Ⅱ. Conclusion The study showed that fatty acids composition ratios of oral fatty loads influenced the dynamic metabolism of the postprandial lipids and lipoproteins metabolism in type Ⅱ diabetic patients. We suggested that the fatty acid composition ratio of 1∶1.7∶1.2 could be used in the fatty load.