Aim To investigate the change of plasma L-arginine, serum NO2 , NO3 and tissue plasminogen activator (t-PA) in patients with coronary heart disease and haperlipemia.Methods The method of Pico-Taq TM amino acid analysis was introduced to measure the level of L-arginine. Serum NO2 and NO3 was observed by catalytic photometric method. Serum t-PA was measured by enzyme linked immunosorbent assay.Results The levels of plasma L-arginine (patients vs control group: 89±8μmol/L vs 113±5 μmol/L),serum superoxide dismutase (patients vs control group: 67 ± 33 ku/L vs 98± 34 ku/L) and tPA (patients vs control group: 0. 94 ±0. 24 ku/L vs 1. 43±0.21 ku/L) were reduced (P〈0. 05 ), but the levels of serum malondialdehyde (patients vs control group: 7. 1 ±1. 5 μmol/L vs 3. 5±0.6μmol/L) and NO2, NO3 (patients vs control group: 38±10μmol/L vs 30± 9 μmol/L) were increased (P〈0. 05).Concluslon Absence of plasma L-arginine in patients heart disease and hyperlipemia may be related with the increase of requirement of nitric oxide and increase of serum NO2 , NO3 may be related with increase of degradation of nitric oxide. The decrease of tPA activity may be related with endothelium disfunction.