Aim The purpose of this paper is to investigate whether the lipid peroxidation of endothelial cells had any effect on the production of growth factors by them.Methods The bovine aortic endothelial cells were isolated and cultured using an enzyme-dispersal method.and the media conditioned by endothelial cells with lipid peroxidation induced by cumene hydroperoxide (LPECCM) were collected. The mitogenic activity of LPECCM for Swiss 3T3 cells was determined by incorporation of[3H]-thymidine into DNA of the cells.The effect of lipid peroxidation of endothelial cells on their synthesis and secretion of PDGF was performed with a neutralization assay using anti-PDGF antibodles and Northern blot analysis. Results Cumene hydroperoxide induced storage of lipid peroxide in cultured endothelial cells, and there was an obvious increase in the incorporation of[3H] thymidine into DNA in wiss 3T3 cells treated with LPECCM compared with the control.However.the mitogenic activity of LPECCM for Swiss 3T3 cells were not inhibited by anti-PDGF-AA and anti-PDGFBB antibodies. Conversely, a weaker PDGF-B chain mRNA expression in endothelial cells was observed after exposure to cumene hydroperoxide.Conclusions It seems reasonable to believe that lipid peroxidation of endothelial cells induced by cumene hydroperoxide leads to an increased synthesis and secretion of growth factors other than PDGF in cultured bovine aortic endothelial cells.